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gift fromkurt beam  (Addgene inc)


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    Structured Review

    Addgene inc gift fromkurt beam
    Gift Fromkurt Beam, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gift+fromkurt+beam/pcDNA3%2E1-Clover-mRuby2+(Plasmid+%2349089)/pm30759393-195-35-38
    Average 93 stars, based on 24 article reviews
    gift fromkurt beam - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Transgenic Assay:

    Article Title: CFTR Protects against Mycobacterium abscessus Infection by Fine-Tuning Host Oxidative Defenses.
    Article Snippet: .. Creation of theTg(mpeg1:NLSmClover) transgenic line The Tol2kit multisite Gateway-based transposon system (Kwan et al., 2007) was used to generated a construct from which a stable transgenic line was raised. mClover was amplified from pcDNA3.1-Clover-mRuby2, a gift fromKurt Beam (Addgene, plasmid 49089), using forward forward primer 50-GGGGACAAGTTTGTACAAAAAAAGGCTCAATGGCTCCAAAGAAGAAGCGTAAGGTA-30 and reverse primer 50-GGGGACCACTTTGTACAAGAAAGCTGGGTCTACTTGTACAGCTCGTCCA-30 and cloned into pDONR 221 donor vector (Invitrogen) to produce pME-NLSmClover. .. An LR Clonase II Plus (Invitrogen) Gateway reaction was performed with p5Empeg1 (Ellett et al., 2011), pME-NLSmClover and p3E-polyA inserted into pDestTol2pA2 destination vector (Kwan et al., 2007) to e2 Cell Reports 26, 1828–1840.e1–e4, February 12, 2019 producempeg1:NLSmClover construct.

    Generated:

    Article Title: CFTR Protects against Mycobacterium abscessus Infection by Fine-Tuning Host Oxidative Defenses.
    Article Snippet: .. Creation of theTg(mpeg1:NLSmClover) transgenic line The Tol2kit multisite Gateway-based transposon system (Kwan et al., 2007) was used to generated a construct from which a stable transgenic line was raised. mClover was amplified from pcDNA3.1-Clover-mRuby2, a gift fromKurt Beam (Addgene, plasmid 49089), using forward forward primer 50-GGGGACAAGTTTGTACAAAAAAAGGCTCAATGGCTCCAAAGAAGAAGCGTAAGGTA-30 and reverse primer 50-GGGGACCACTTTGTACAAGAAAGCTGGGTCTACTTGTACAGCTCGTCCA-30 and cloned into pDONR 221 donor vector (Invitrogen) to produce pME-NLSmClover. .. An LR Clonase II Plus (Invitrogen) Gateway reaction was performed with p5Empeg1 (Ellett et al., 2011), pME-NLSmClover and p3E-polyA inserted into pDestTol2pA2 destination vector (Kwan et al., 2007) to e2 Cell Reports 26, 1828–1840.e1–e4, February 12, 2019 producempeg1:NLSmClover construct.

    Construct:

    Article Title: CFTR Protects against Mycobacterium abscessus Infection by Fine-Tuning Host Oxidative Defenses.
    Article Snippet: .. Creation of theTg(mpeg1:NLSmClover) transgenic line The Tol2kit multisite Gateway-based transposon system (Kwan et al., 2007) was used to generated a construct from which a stable transgenic line was raised. mClover was amplified from pcDNA3.1-Clover-mRuby2, a gift fromKurt Beam (Addgene, plasmid 49089), using forward forward primer 50-GGGGACAAGTTTGTACAAAAAAAGGCTCAATGGCTCCAAAGAAGAAGCGTAAGGTA-30 and reverse primer 50-GGGGACCACTTTGTACAAGAAAGCTGGGTCTACTTGTACAGCTCGTCCA-30 and cloned into pDONR 221 donor vector (Invitrogen) to produce pME-NLSmClover. .. An LR Clonase II Plus (Invitrogen) Gateway reaction was performed with p5Empeg1 (Ellett et al., 2011), pME-NLSmClover and p3E-polyA inserted into pDestTol2pA2 destination vector (Kwan et al., 2007) to e2 Cell Reports 26, 1828–1840.e1–e4, February 12, 2019 producempeg1:NLSmClover construct.

    Amplification:

    Article Title: CFTR Protects against Mycobacterium abscessus Infection by Fine-Tuning Host Oxidative Defenses.
    Article Snippet: .. Creation of theTg(mpeg1:NLSmClover) transgenic line The Tol2kit multisite Gateway-based transposon system (Kwan et al., 2007) was used to generated a construct from which a stable transgenic line was raised. mClover was amplified from pcDNA3.1-Clover-mRuby2, a gift fromKurt Beam (Addgene, plasmid 49089), using forward forward primer 50-GGGGACAAGTTTGTACAAAAAAAGGCTCAATGGCTCCAAAGAAGAAGCGTAAGGTA-30 and reverse primer 50-GGGGACCACTTTGTACAAGAAAGCTGGGTCTACTTGTACAGCTCGTCCA-30 and cloned into pDONR 221 donor vector (Invitrogen) to produce pME-NLSmClover. .. An LR Clonase II Plus (Invitrogen) Gateway reaction was performed with p5Empeg1 (Ellett et al., 2011), pME-NLSmClover and p3E-polyA inserted into pDestTol2pA2 destination vector (Kwan et al., 2007) to e2 Cell Reports 26, 1828–1840.e1–e4, February 12, 2019 producempeg1:NLSmClover construct.

    Plasmid Preparation:

    Article Title: CFTR Protects against Mycobacterium abscessus Infection by Fine-Tuning Host Oxidative Defenses.
    Article Snippet: .. Creation of theTg(mpeg1:NLSmClover) transgenic line The Tol2kit multisite Gateway-based transposon system (Kwan et al., 2007) was used to generated a construct from which a stable transgenic line was raised. mClover was amplified from pcDNA3.1-Clover-mRuby2, a gift fromKurt Beam (Addgene, plasmid 49089), using forward forward primer 50-GGGGACAAGTTTGTACAAAAAAAGGCTCAATGGCTCCAAAGAAGAAGCGTAAGGTA-30 and reverse primer 50-GGGGACCACTTTGTACAAGAAAGCTGGGTCTACTTGTACAGCTCGTCCA-30 and cloned into pDONR 221 donor vector (Invitrogen) to produce pME-NLSmClover. .. An LR Clonase II Plus (Invitrogen) Gateway reaction was performed with p5Empeg1 (Ellett et al., 2011), pME-NLSmClover and p3E-polyA inserted into pDestTol2pA2 destination vector (Kwan et al., 2007) to e2 Cell Reports 26, 1828–1840.e1–e4, February 12, 2019 producempeg1:NLSmClover construct.

    Clone Assay:

    Article Title: CFTR Protects against Mycobacterium abscessus Infection by Fine-Tuning Host Oxidative Defenses.
    Article Snippet: .. Creation of theTg(mpeg1:NLSmClover) transgenic line The Tol2kit multisite Gateway-based transposon system (Kwan et al., 2007) was used to generated a construct from which a stable transgenic line was raised. mClover was amplified from pcDNA3.1-Clover-mRuby2, a gift fromKurt Beam (Addgene, plasmid 49089), using forward forward primer 50-GGGGACAAGTTTGTACAAAAAAAGGCTCAATGGCTCCAAAGAAGAAGCGTAAGGTA-30 and reverse primer 50-GGGGACCACTTTGTACAAGAAAGCTGGGTCTACTTGTACAGCTCGTCCA-30 and cloned into pDONR 221 donor vector (Invitrogen) to produce pME-NLSmClover. .. An LR Clonase II Plus (Invitrogen) Gateway reaction was performed with p5Empeg1 (Ellett et al., 2011), pME-NLSmClover and p3E-polyA inserted into pDestTol2pA2 destination vector (Kwan et al., 2007) to e2 Cell Reports 26, 1828–1840.e1–e4, February 12, 2019 producempeg1:NLSmClover construct.



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    gift fromkurt beam  (Addgene inc)
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    Addgene inc gift fromkurt beam
    Gift Fromkurt Beam, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gift+fromkurt+beam/pcDNA3%2E1-Clover-mRuby2+(Plasmid+%2349089)/pm30759393-195-35-38
    Average 93 stars, based on 1 article reviews
    gift fromkurt beam - by Bioz Stars, 2026-10
    93/100 stars
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